In the course of our experiment we were investigating the toxicity of different forms of selenium on HaCaT cell cultures in vitro. The forms were:  selenate, selenite, biological and chemical selenium forms and nanosized selenium. 
    During our study the toxicity of different forms of selenium was monitored on HaCaT – human keratinocyte – cell cultures depending on the chromatin condensation. We also used controll cultures for our assay, to which we did not use any kind of selenium. We added to the cell cultures solutions of four different concentrations of selenium: 1ppm, 5ppm, 10ppm and 50ppm. To study the chromatin condensation we bred the cell cultures, after that we isolated the cell nuclei then with the help of fluorescent microscopy we observated the cell nuclei. 
    According to the results we can say that the less toxic was the biological selenium form while the selenate proved to be the most toxic selenium form. 

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Selenite

 
Selenate

Biological selenium 5ppm

Chemical selenium 50ppm