Our projects concentrating on the development of new technologies

 

perfúziós fotó jpeg

 

 

 

 

The system is based on Long-Term scanning microscopy developed by the eTox Workgroup in 2005. With the use of LTS a digital photo of the cell culture is taken in every minute and after the experiment the sequence of photos is converted to a video which is analyzable along quantitative and qualitive parameters. With this technique it’s possible to monitor the cells in the whole time of the experiment and make a long-term light microscopic observation of the cell culture at population level or individual cells.

 

The LTS microscopy supported by perfusion system allows to change the cell culture media during the examination or add chemicals to the culture without moving the cell culture flask and interrupting the observation. So the morphological and behavioural changes of specific regions under chemical agents become noticable. We can stay clear of the physical contact with the culture with the use of the perfusion system, so it provides protection againts contamination, too.

 

The perfusion system is pneumatically driven, the change of the media is carried out by cylinders and syringes moved by air pressure and vacuum. The whole system can be controlled from outside of the incubator. The system contains all of the solutions needed for the experiment before the observation begins. They are located in the syringes fixed in the cylinders in the incubator. The injection of the solutions to the cell culture flask is done through special needles. 

 

 Changes of numbers of mitotic cells in a 24 h serum-starved HaCaT cell culture - the diagram is made by the analysis of the photo sequence:

 

24 h serum-starvation diagram

 

 

 

 

 

 

 

 

 

Effects of sodium-butyrate on HaCaT cells: the video shows the cells at the presence of the histon-deacetylase inhibitor and the instantaneous behavioural effects of the removing of the chemical:

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Our old 3D reconstruction of a silver-impregnated neuron.

 

N E U R O N by bigdegu on Sketchfab

 

 

Attached presentation in hungarian:

Families in USA buy more medicines per person than any other area. The availability of remedies provided by companies is certainly a contributing matter to prescription treatment abuse epidemic. Many patients already know that medications are made to treat health problems, but they can hurt us if taken incorrectly. Cialis is a well-known generic used to treat inability to get or keep an erection. Topics such as "how to get a free trial of viagra" or "generic name for viagra" are very pop now. Perhaps you already heard about it. What scientists talk about "what is the generic name for viagra"? Other question we should is "healthy man viagra". Sometimes men who take street drugs like marijuana find it awkward to get an erection and turn to prescription drugs for a temporary solution. Additionally there are various unproven herbal medicines for erectile malfunction.

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